Mouse Macrophage Inflammatory Protein 3 Alpha ELISA Kit from Innovative Research is intended for the quantitative determination of Mouse Macrophage Inflammatory Protein 3 Alpha in biofluid samples, such as serum, plasma and other biological fluids. This is a sandwich ELISA in strip well format with reagents for up to 96 tests . MIP3a present in samples reacts with an anti-Mouse MIP3a capture antibody coated and dried on a microtiter plate. After appropriate washing steps, an anti-MIP3a detection antibody conjugated to biotin binds to the captured analyte. Excess antibody is washed away and bound antibody is then reacted with avidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. Color development is directly proportional to the concentration of active MIP3a in the samples. A standard calibration curve is prepared along with the samples to be measured using dilutions of Mouse MIP3a. Detection Target: Mouse Macrophage Inflammatory Protein 3 Alpha Reactivity: Mouse Range: 78.1-5000pg/mL Sensitivity: 27pg/mL Storage Conditions: Store all components at 4┬░C This Mouse MIP3a activity assay is for the quantitative determination of Macrophage Inflammatory Protein 3 Alpha in samples including serum, plasma and other biological fluids. For research use only. Strip well format. Reagents for up to 96 tests. For research use only. At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.